ITGAM Antibody : FITC (OATA00199)

Catalog No: OATA00199

Human peripheral blood monocytes were stained with 5 uL (1 ug) FITC Anti-Human CD11b (OATA00199) (solid line) or 1 ug FITC Mouse IgG1 isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus, Swine
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: ICRF44
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 520 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD11b
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Feng C, Zhang L, Almulki L, Faez S, Whitford M, Hafezi-Moghadam A, and Cross AS. 2011. J. Leukoc. Biol. 90:313-321. (Immunoprecipitation)

  Chang WLW and Barry PA. 2010. Proc. Natl. Acad. Sci. 107:22647-2652. (Flow cytometry - Rhesus macaque)

  Jerke U, Rolle S, Dittmar G, Bayat B, Santoso S, Sporbert A, Luft F, and Kettritz R. 2010. J. Biol. Chem. 286:7070-7081. (in vitro blocking)

  Moreau A, Hill M, Thebault P, Deschamps JY, Chiffoleau E, Chauveau C, Moullier P, Anegon I, Alliot-Licht B, and Cuturi MC. 2009. FASEB J. 23:3070-3077. (Flow cytometry - cynomolgus macaque)

  Sengoku K, Takuma N, Miyamoto T, Horikawa M, and Ishikawa M. 2004. Hum. Reprod. 19: 639-644. (Immunofluorescence microscopy)

  David A, Kacher Y, Specks U, and Aviram I. 2003. J. Leukoc. Biol. 74:551-557. (Western blot)Rezzonico R, Imbert V, Chicheportiche R, and Dayer J-M. 2001. Blood. 97: 2932-2940. (in vitro activation)

Target Info

• Gene Symbol: ITGAM
• Gene Full Name: integrin subunit alpha M
• Alias Symbols: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6
• NCBI Gene Id: 3684; 454069; 101866035
• Protein Name: integrin alpha-M
• Description of Target: This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P11215
• Protein Accession #: NP_000623.2
• Nucleotide Accession #: NM_000632.3