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Catalog No: OKEH01078
Size:96T
Price: $525.00
SKU
OKEH01078
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ITGA4 ELISA Kit (Human) (OKEH01078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITGA4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITGA4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITGA4 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.7% (n = 20)
Mean Inter-assay CV%: < 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
ITGA4 Microplate96 Wells (12 x 8 Well strips)
ITGA4 Lyophilized Standard2
100X Biotinylated ITGA4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Integrin alpha-4
Publications

AGT haplotype in ITGA4 gene is related to antibody-mediated rejection in heart transplant patients. PLoS One. 14, e0219345 (2019). 31335901

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolITGA4
Gene Full Nameintegrin subunit alpha 4
Alias Symbols269C wild type;alpha 4 subunit of VLA-4 receptor;antigen CD49D, alpha-4 subunit of VLA-4 receptor;CD49 antigen-like family member D;CD49D;IA4;integrin alpha-4;integrin alpha-IV;very late activation protein 4 receptor, alpha 4 subunit;VLA-4 subunit alpha.
NCBI Gene Id3676
Protein NameP13612
Description of TargetIntegrin alpha-4
Uniprot IDhttps://www.uniprot.org/uniprot/P13612
Protein Accession #NP_000876.3
Nucleotide Accession #NM_000885.5
Protein Size (# AA)1032
Molecular Weight115 kDa
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