ITGA2B ELISA Kit (Human) (OKCD06583)

Catalog No: OKCD06583

Size:96WELLS

ITGA2B ELISA Kit (Human) (OKCD06583) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glycoprotein IIb/IIIa (GPIIb/IIIa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glycoprotein IIb/IIIa (GPIIb/IIIa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glycoprotein IIb/IIIa (GPIIb/IIIa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Glycoprotein IIb/IIIa (GPIIb/IIIa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycoprotein IIb/IIIa (GPIIb/IIIa) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycoprotein IIb/IIIa (GPIIb/IIIa) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ITGA2B Microplate: 96 Wells (12 x 8 Well strips)
  • ITGA2B Lyophilized Standard: 2
  • 100X ITGA2B HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.057ng/mL

Target Info

• Gene Symbol: ITGA2B
• Gene Full Name: integrin subunit alpha 2b
• Alias Symbols: alphaIIb protein;BDPLT16;BDPLT2;CD41;CD41B;GP2B;GPalpha IIb;GPIIb;GT;GT1;GTA;HPA3;integrin alpha-IIb;integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41);platelet fibrinogen receptor, alpha subunit;platelet glycoprotein IIb of IIb/IIIa complex;platelet membrane glycoprotein IIb;platelet-specific antigen BAK;PPP1R93;protein phosphatase 1, regulatory subunit 93.
• NCBI Gene Id: 3674
• Protein Name: Integrin alpha-IIb
• Description of Target: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
• Uniprot ID: P08514
• Protein Accession #: NP_000410.2
• Nucleotide Accession #: NM_000419.4