ITA6 Antibody (OAAB06671)

Catalog No: OAAB06671

Size:400ul

Western blot analysis of anti-ITA6 (Cat. #OAAB06671) in HepG2 cell line lysates (35ug/lane). ITA6 (arrow) was detected using the purified Mab.(8ug/ml)

ITA6 Monoclonal Antibody (Cat. #OAAB06671) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ITA6 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.

ITA6 Monoclonal Antibody (Cat. #OAAB06671) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ITA6 Antibody (Cat#OAAB06671) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Clonality: Monoclonal
• Clone: 129CT49.6.5
• Isotype: IgG1
• Host: Mouse
• Application: WB, IHC, IF, FC
• Additional Information: Precautions: ITA6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome
  Cellular Location: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: ITA6 recombinant protein is used to produce this monoclonal antibody.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IHC-P: 1:10~50
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: ITGA6
• Alias Symbols: CD49f, VLA-6, ITGA6B
• NCBI Gene Id: 3655
• Protein Name: Integrin alpha-6
• Description of Target: Purified Mouse Monoclonal Antibody (Mab)
• Uniprot ID: P23229
• Protein Accession #: NP_000201.2
• Molecular Weight: 126633 Da