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Catalog No: OKCD02655
Size:96WELLS
Price: $700.00
SKU
OKCD02655
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Datasheets/ManualsPrintable datasheet for IRS1 ELISA Kit (Mouse) (OKCD02655)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor Substrate 1 (IRS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor Substrate 1 (IRS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor Substrate 1 (IRS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Receptor Substrate 1 (IRS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor Substrate 1 (IRS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor Substrate 1 (IRS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IRS1 Microplate96 Wells (12 x 8 Well strips)
IRS1 Lyophilized Standard2
100X Biotinylated IRS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.128 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin Receptor Substrate 1 (IRS1). No significant cross-reactivity or interference between Insulin Receptor Substrate 1 (IRS1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolIrs1
Gene Full Nameinsulin receptor substrate 1
Alias SymbolsG972;G972R;insulin receptor substrate 1;IR;IRS-1.
NCBI Gene Id16367
Protein NameInsulin receptor substrate 1
Description of TargetMay mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
Uniprot IDP35569
Protein Accession #NP_034700.2
Nucleotide Accession #NM_010570.4
Protein Size (# AA)1233
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