Catalog No: OKCD04470
Size:96WELLS
Price: $700.00
SKU
OKCD04470
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for IRF4 ELISA kit (Rat)
Product Info
Predicted Species ReactivityRat
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Regulatory Factor 4 (IRF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Regulatory Factor 4 (IRF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Regulatory Factor 4 (IRF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Regulatory Factor 4 (IRF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average (%)
Serum (n=5)82-9792
EDTA Plasma (n=5)84-9389
Heparin Plasma (n=5)95-105101
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Regulatory Factor 4 (IRF4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Regulatory Factor 4 (IRF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Pedf Microplate96 Wells (12 x 8 Well strips)
Pedf Lyophilized Standard2
100X Pedf HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity0.133 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIRF4
NCBI Gene Id291100
Protein Nameinterferon regulatory factor 4
Uniprot IDD3ZEX6
Protein Accession #NP_001099578.1
Nucleotide Accession #NM_001106108.1
Write Your Own Review
You're reviewing:IRF4 ELISA Kit (Rat) (OKCD04470)
Your Rating
We found other products you might like!