IRF3 ELISA Kit (Bovine) (OKEH08065)

Catalog No: OKEH08065

Size:96T

Bovine IRF3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology IRF3 ELISA Kit (Bovine) (OKEH08065) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IRF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IRF3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.1% (n = 20)
  Mean Inter-assay CV%: <=9.1% (n = 20)
• ELISA Kit Component:
  • IRF3 Microplate: 96 Wells (12 x 8 Well strips)
  • IRF3 Lyophilized Standard: 2
  • 100X Biotinylated IRF3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
  Subcellular Location: Cytoplasm Nucleus Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  Unigene: Bt.45332
  SMR: Q4JF28
  String: 9913.ENSBTAP00000031815
  KEGG: bta:516979
  Pfam: PF00605
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.055ng/mL
• Specificity: Natural and recombinant Bovine Interferon regulatory factor 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IRF3
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: interferon regulatory factor 3;IRF-3.
• NCBI Gene Id: 516979
• Protein Name: Q4JF28
• Description of Target: Interferon regulatory factor 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q4JF28
• Protein Accession #: NP_001025016.1
• Nucleotide Accession #: NM_001029845.3