IRF3 Antibody (Phospho-Ser385) (OASG03961)

Catalog No: OASG03961

Size:100 uL

Western Blot using 293

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC, IF
  Cellular Localization: Cytoplasm Nucleus Note=Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
  Tissue Specificity: Expressed constitutively in a variety of tissues.
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human IRF-3 around the phosphorylation site of S385. Location: 320-400aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser385
• Specificity: Phospho-IRF-3 (S385) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S385.
• Application Info: WB: 1:500~2000
  IHC: 1:100~300
  IF: 1:200~1000
  ELISA: 1:10000

Target Info

• Gene Symbol: IRF3
• Gene Full Name: interferon regulatory factor 3
• Alias Symbols: IIAE7
• NCBI Gene Id: 3661;
• Description of Target: IRF-3 is a protein encoded by the IRF3 gene which is approximately 47,2 kDa. IRF-3 is localised to the cytoplasm and nucleus. It is involved in activated TLR4 signalling and toll-like receptor signalling pathway. This protein falls under the interferon regulatory transcription factor family. It is found in an inactive form in the cytoplasm that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex is then translocated to the nucleus where it activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. IRF-3 is expressed constitutively in a variety of tissues. Mutations in the IRF3 gene may be involved in herpes simplex encephalitis. STJ91040 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous IRF-3 protein which only binds about S385 when S385 is phosphorylated.
• Uniprot ID: Q14653
• Molecular Weight: 60 kDa