IRF1 Antibody (OACD04839)

Catalog No: OACD04839

Western Blot with Mouse Recombinant Interferon regulatory factor 1

Western Blot with Mouse Recombinant Interferon regulatory factor 1

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Range: His44 - Pro319
• Purification: Affinity Purified; Protein A Purified
• Concentration: 0.5 mg/ml
• Preservative: 0.02% NaN3
• Specificity: The antibody is a rabbit polyclonal antibody raised against IRF1. It has been selected for its ability to recognize IRF1 in immunohistochemical staining and western blotting.
• Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
• Application Data: Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-1:5,000
  Optimal working dilutions must be determined by end user.

Target Info

• Gene Symbol: Irf1
• Gene Full Name: interferon regulatory factor 1
• Alias Symbols: AU020929;interferon regulatory factor 1;Irf;Irf-1.
• NCBI Gene Id: 16362
• Protein Name: Interferon regulatory factor 1
• Description of Target: Transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. These include the regulation of IFN and IFN-inducible genes, host response to viral and bacterial infections, regulation of many genes expressed during hematopoiesis, inflammation, immune responses and cell proliferation and differentiation, regulation of the cell cycle and induction of growth arrest and programmed cell death following DNA damage. Stimulates both innate and acquired immune responses through the activation of specific target genes and can act as a transcriptional activator and repressor regulating target genes by binding to an interferon-stimulated response element (ISRE) in their promoters. Its target genes for transcriptional activation activity are: genes involved in anti-viral response, such as IFN-alpha/beta, DDX58/RIG-I, TNFSF10/TRAIL, OAS1/2, PIAS1/GBP, EIF2AK2/PKR and RSAD2/viperin; antibacterial response, such as NOS2/INOS; anti-proliferative response, such as p53/TP53, LOX and CDKN1A; apoptosis, such as BBC3/PUMA, CASP1, CASP7 and CASP8; immune response, such as IL7, IL12A/B and IL15, PTGS2/COX2 and CYBB; DNA damage responses and DNA repair, such as POLQ/POLH; MHC class I expression, such as TAP1, PSMB9/LMP2, PSME1/PA28A, PSME2/PA28B and B2M and MHC class II expression, such as CIITA. Represses genes involved in anti-proliferative response, such as BIRC5/survivin, CCNB1, CCNE1, CDK1, CDK2 and CDK4 and in immune response, such as FOXP3, IL4, ANXA2 and TLR4. Stimulates p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Plays an important role in immune response directly affecting NK maturation and activity, macrophage production of IL12, Th1 development and maturation of CD8+ T-cells. Also implicated in the differentiation and maturation of dendritic cells and in the suppression of regulatory T (Treg) cells development. Acts as a tumor suppressor and plays a role not only in antagonism of tumor cell growth but also in stimulating an immune response against tumor cells.
• Uniprot ID: P15314
• Protein Accession #: NP_001152868.1
• Nucleotide Accession #: NM_001159396
• Molecular Weight: 37 kDa