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| Datasheets/Manuals | Printable datasheet for IRAK4 Antibody (Phospho-Thr345) (OABF00384) |
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| Predicted Species Reactivity | Bovine|Dog|Guinea Pig|Human|Mouse|Porcine|Rabbit|Rat|Sheep |
|---|---|
| Product Format | Liquid. 1% BSA, 50% glycerol and 0.09% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot |
| Additional Information | Subcellular location: Cytoplasm Modification: Phosphorylation (Thr345) |
| Reconstitution and Storage | -20°C |
| Immunogen | KLH conjugated Synthesised phosphopeptide derived from human IRAK4 around the phosphorylation site of Thr345 |
| Purification | Protein A Purified |
| Concentration | 1 ug/ul |
| Application Info | WB 1:500-1000 IHC-P 1:100-500 Fluorescent IHC 1:50-200 |
| Gene Symbol | IRAK4 |
|---|---|
| Gene Full Name | interleukin 1 receptor associated kinase 4 |
| Alias Symbols | IMD67;interleukin-1 receptor-associated kinase 4;IPD1;IRAK-4;NY-REN-64;REN64;renal carcinoma antigen NY-REN-64. |
| NCBI Gene Id | 51135 |
| Protein Name | Interleukin-1 receptor-associated kinase 4 |
| Description of Target | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections. |
| Uniprot ID | Q9NWZ3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Bovine|Dog|Guinea Pig|Human|Mouse|Porcine|Rabbit|Rat|Sheep".
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How long will it take to receive "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
This item is available "3 to 5 business days.".
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What buffer format is "IRAK4 Antibody (Phospho-Thr345) (OABF00384)" provided in?
This item is provided in "Liquid. 1% BSA, 50% glycerol and 0.09% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
This target may also be called "IMD67;interleukin-1 receptor-associated kinase 4;IPD1;IRAK-4;NY-REN-64;REN64;renal carcinoma antigen NY-REN-64." in publications.
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What is the shipping cost for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "IRAK4 Antibody (Phospho-Thr345) (OABF00384)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IRAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IRAK4"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IRAK4"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IRAK4"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IRAK4"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IRAK4"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
