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Catalog No: OKCD01275 (Formerly GWB-KBBDL5)
Size:96WELLS
Price: $675.00
SKU
OKCD01275
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Datasheets/ManualsPrintable datasheet for IRAK2 ELISA Kit (Human) (OKCD01275)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 2 (IRAK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 2 (IRAK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 2 (IRAK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 2 (IRAK2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-IRAK2 Microplate96 Wells (12 x 8 Well Strips)
IRAK2 Lyophilized Standard2 x 50 ng
100X Biotinylated IRAK2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.27 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 2 (IRAK2). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 2 (IRAK2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIRAK2
Gene Full Nameinterleukin 1 receptor associated kinase 2
Alias Symbolsinterleukin-1 receptor-associated kinase-like 2;IRAK-2.
NCBI Gene Id3656
Protein NameInterleukin-1 receptor-associated kinase-like 2
Description of TargetBinds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
Uniprot IDO43187
Protein Accession #NP_001561
Nucleotide Accession #NM_001570.3
Protein Size (# AA)625
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