IQGAP2 ELISA Kit (Mouse) (OKCD08628)

Catalog No: OKCD08628

Size:96WELLS

IQGAP2 ELISA Kit (Mouse) (OKCD08628) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IQ Motif Containing GTPase Activating Protein 2 (IQGAP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IQ Motif Containing GTPase Activating Protein 2 (IQGAP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IQ Motif Containing GTPase Activating Protein 2 (IQGAP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IQ Motif Containing GTPase Activating Protein 2 (IQGAP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IQGAP2 Microplate: 96 Wells (12 x 8 Well strips)
  • IQGAP2 Lyophilized Standard: 2
  • 100X IQGAP2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.062ng/mL

Target Info

• Gene Symbol: IQGAP2
• Gene Full Name: IQ motif containing GTPase activating protein 2
• Alias Symbols: AI788777, A630053O10, 4933417J23Rik
• NCBI Gene Id: 544963
• Protein Name: ras GTPase-activating-like protein IQGAP2
• Description of Target: Binds to activated cdc42 and rac1 but does not seem to stimulate their gtpase activity. associates with calmodulin.
• Uniprot ID: Q3UQ44
• Protein Accession #: NP_081987.1
• Nucleotide Accession #: NM_027711.1