INTERLEUKIN-2 Recombinant Protein (OPSA10945)
This product is Genway GWB-8EBABD
|Predicted Species Reactivity||Human|
|Product Format||Purified protein produced in E. coli - lyophilised|
|Additional Information||Activity: 1 x 107 units/mg
Approx Protein Conc: Total protein concentration 0.1 mg/ml after reconstitution
|::||Endotoxin Level: < 0.1 ng/ug|
|::||Preservative: None present
Buffer Solution: 10mM Sodium citrate
|Reconstitution and Storage||Reconstitute with 0.5 ml 0.1M acetic acid
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Aviva Systems Biology recommend that the vial is gently mixed after reconstitution.
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 3 months from date of reconstitution.
|Predicted Homology Based on Immunogen Sequence||Human|
|Purity||>97% by SDS PAGE and HPLC analysis|
|Application Info||ELISA: 0.2 - 0.4ng/well
This product may be used as a standard for ELISA applications with either OASA07877 or OASA07879
Western Blotting: 1.5 - 3.0ng/lane
This product may be used as a positive control for Western Blotting applications with either OASA07877 or OASA07879
|Target Reference||1. Akari, H. et al. (2000) Nef-induced major histocompatibility complex class I down-regulation is functionally dissociated from its virion incorporation, enhancement of viral infectivity, and CD4 down-regulation. J. Virol. 74 (6): 2907 - 2912.
2. Chan, P.K. et al. (2010) T-cell response to human papillomavirus type 58 L1, E6 and E7 peptides in women with cleared infection, cervical intraepithelial neoplasia and invasive cancer.
|Datasheets/Manuals||Printable datasheet for OPSA10945|
|Alias Symbols||IL-2, TCGF, lymphokine, IL2|
|NCBI Gene Id||3558|
|Description of Target||RECOMBINANT HUMAN INTERLEUKIN-2
|Protein Accession #||NP_000577.2|
|Protein Size (# AA)||15.4kD (134 Amino acid sequence)|
|Tissue Tool||Find tissues and cell lines supported by DNA array analysis to express IL2.|
|RNA Seq||Find tissues and cell lines supported by RNA-seq analysis to express IL2.|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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