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Catalog No: OKCD00165 (Formerly GWB-KBBMA6)
Size:96WELLS
Price: $450.00
SKU
OKCD00165
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Datasheets/ManualsPrintable datasheet for INTERLEUKIN 1-BETA ELISA Kit (Pig) (OKCD00165)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)84-98%80-90%78-98%86-99%
EDTA plasma(n=5)99-105%86-95%95-105%78-99%
heparin plasma(n=5)99-105%91-104%82-102%99-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Beta (IL1b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Beta (IL1b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Beta (IL1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Beta (IL1b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)93-10598
EDTA plasma(n=5)97-105101
heparin plasma(n=5)90-9793
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Beta (IL1b) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-interleukin 1-beta Microplate96 Wells (12 x 8 Well Strips)
interleukin 1-beta Lyophilized Standard2 x 1 ng
100X Biotinylated interleukin 1-beta Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 0.64 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Beta (IL1b). No significant cross-reactivity or interference between Interleukin 1 Beta (IL1b) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolIL1B2
Gene Full Nameinterleukin 1, beta 2
Alias SymbolsIL1B;IL-1beta;interleukin 1, beta;interleukin-1 beta.
NCBI Gene Id396565
Protein NameMultifunctional fusion protein
Description of TargetPotent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
Uniprot IDQ29082
Protein Accession #NP_001005149
Nucleotide Accession #NM_001005149.1
Protein Size (# AA)267
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