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Catalog No: OKCD00159 (Formerly GWB-KBBGP3)
Size:96WELLS
Price: $725.00
SKU
OKCD00159
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Datasheets/ManualsPrintable datasheet for INTERFERON-BETA-1 ELISA Kit (Pig) (OKCD00159)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)91-104%78-99%86-102%79-104%
EDTA plasma(n=5)78-94%80-96%98-105%83-104%
heparin plasma(n=5)79-99%99-105%80-99%79-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta (IFNb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interferon Beta (IFNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta (IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interferon Beta (IFNb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-9693
EDTA plasma(n=5)85-9288
heparin plasma(n=5)78-10598
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-interferon-beta-1 Microplate96 Wells (12 x 8 Well Strips)
interferon-beta-1 Lyophilized Standard2 x 1 ng
100X Biotinylated interferon-beta-1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 5.6 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb). No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolINTERFERON-BETA-1
Alias SymbolsIFNb, IFN-beta
NCBI Gene Id445459
Protein NameInterferon beta precursor
Description of TargetInterferon beta precursor
Uniprot IDQ29412
Protein Accession #NP_001003923
Nucleotide Accession #NM_001003923.1
Protein Size (# AA)186
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