Insulin Auto-Antibody ELISA Kit (Human) (OKCD01296)

Catalog No: OKCD01296

Size:96WELLS

Human Insulin Auto-antibody ELISA Kit Kit (Human) (OKCD01296) ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 2h, 30min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-105%	96-103%	95-102%	88-101%
  EDTA Plasma (n=5)	79-93%	81-101%	91-99%	95-103%
  Heparin Plasma (n=5)	96-105%	78-99%	87-96%	94-101%

• ELISA Kit Principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Insulin Autoantibody (IAA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Insulin Autoantibody (IAA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	102
  EDTA Plasma (n=5)	83-96	86
  Heparin Plasma (n=5)	92-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Autoantibody (IAA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Insulin Auto-antibody Microplate: 96 Wells (12 x 8 Well strips)
  • Insulin Auto-antibody Lyophilized Standard: 2 x XXX
  • 100X Detector Antibody: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 1.15 ng/mL
• Assay Info: Assay Method: Quantitative Reverse Capture Sandwich ELISA

Target Info

• Alias Symbols: INSR, IDDM2, ILPR, Insulin, IRDN, MODY10
• Protein Name: Insulin Autoantibody
• Description of Target: Insulin Auto-antibody
• Uniprot ID: P01308
• Nucleotide Accession #: NM_000207.2