INSIG1 Antibody - middle region (ARP40166_P050)

Catalog No: ARP40166_P050

WB Suggested Anti-INSIG1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Muscle

Host: Rabbit
Target Name: WT1
Sample Type: 721_B
Antibody Dilution: 1.0ug/mlINSIG2 is supported by BioGPS gene expression data to be expressed in 721_B

Host: Rabbit
Target: INSIG2
Positive control (+): Human stomach (ST)
Negative control (-): MCF7 (N10)
Antibody concentration: 1ug/ml

Host: Rabbit
Target Name: INSIG1
Sample Tissue: Human HT1080 Whole Cell
Antibody Dilution: 3ug/ml

Host: Rabbit
Target Name: INSIG1
Sample Tissue: Human OVCAR-3 Whole Cell
Antibody Dilution: 2ug/ml

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Pig, Rabbit, Sheep, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INSIG1
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 87%
• Peptide Sequence: Synthetic peptide located within the following region: WWVPPCCGTASAVIGLLYPCIDRHLGEPHKFKREWSSVMRCVAVFVGINH
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-INSIG2 (ARP40166_P050) antibody is Catalog # AAP40166 (Previous Catalog # AAPP20909)
• Sample Type Confirmation:

  INSIG2 is supported by BioGPS gene expression data to be expressed in 721_B

• Reference: Li,C.G., (2008) Int. J. Cancer 123 (2), 273-282
• Description:
  View product citations for INSIG1 Antibody - middle region (ARP40166_P050) on CiteAb

Target Info

• Gene Symbol: INSIG2
• Gene Full Name: Insulin induced gene 2
• Alias Symbols: INSIG-2
• NCBI Gene Id: 51141
• Protein Name: Insulin-induced gene 2 protein
• Description of Target: Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. INSIG1 is an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. INSIG1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins.The protein encoded by this gene is highly similar to the protein product encoded by gene INSIG1. Both INSIG1 protein and this protein are endoplasmic reticulum proteins that block the processing of sterol regulatory element binding proteins (SREBPs) by binding to SREBP cleavage-activating protein (SCAP), and thus prevent SCAP from escorting SREBPs to the Golgi. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Uniprot ID: Q9Y5U4
• Protein Accession #: NP_057217
• Nucleotide Accession #: NM_016133
• Protein Size (# AA): 225
• Molecular Weight: 25kDa
• Protein Interactions: RNF5; HMGCR; SCAP; SREBF1; RNF139; UBQLN1; UBQLN2; FAF2; ADRM1; PSMD14; BAG6; VCP; UBC; RPN1; PSMD13; PSMD12; PSMD11; PSMD4; PSMC6; PSMC5; PSMC3; PSMC2; PSMB3; PSMB1; PSMA7; PSMA2; LGALS7; JUP; INSIG1; HSPA8; HSPA6; HSPA5; HSPA1A; ENO1; DSP; AMFR; SREBF2;