Catalog No: OKEH02984
Size:96T
Price: $425.00
SKU
OKEH02984
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology INS ELISA Kit (Mouse) (OKEH02984) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse INS antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated INS and incubated. The INS found in the sample or standards competes with the biotinylated INS for limited binding sites on the immobilized anti-mouse INS antibody. Excess unbound biotinylated INS and sample or standard INS is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated INS captured in the well and inversely proportional to the amount of INS which was contained in the sample or standard.
ELISA Kit Range0.625-40mIU/L
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 7.5% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-INS Microplate96 Wells (12 x 8 Well strips)
INS Lyophilized Standard2
Sample Diluent1 x 20 mL
100X INS-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.312mIU/L
SpecificityNatural and recombinant Mouse Insulin-1
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolIns1
Gene Full Nameinsulin I
Alias SymbolsIns-1;Ins2-rs1;insulin-1.
NCBI Gene Id16333
Protein NameP01325
Description of TargetInsulin-1
Uniprot IDhttps://www.uniprot.org/uniprot/P01325
Protein Accession #NP_032412.3
Nucleotide Accession #NM_008386.4
Protein Size (# AA)108
Molecular Weight12 kDa
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