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INS ELISA Kit (Chicken) (OKEH07010)

96 Wells
$659.00
In Stock
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Gene Symbol:
INS
NCBI Gene Id:
396145
Alias Symbols:
Insulin
Description of Target:
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Protein Name:
Integrin beta-1
Swissprot Id:
P67970
Protein Accession #:
NP_990553
Nucleotide Accession #:
NM_205222
Predicted Species Reactivity:
Chicken
Sample Type:
Serum, Plasma, Tissue Homogenates
Sensitivity:
0.625-40 uIU/mL
Kit Range:
0.625 - 40 uIU/mL
Kit Reproducibility:
Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Kit Duration:
~3 Hours
Kit Principle:
Aviva Systems Biology Ins ELISA Kit (Chicken) (OKEH07010) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ins has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ins is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ins captured in the well.
Kit Component:
ComponentAmount
Ins Microplate96 Wells (12 x 8 Well strips)
Ins Lyophilized Standard2 x 40 uIU
100X Ins HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Kit Detection Method:
Colorimetric, OD 450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express INS.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express INS.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA

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