INPP5D Recombinant Antibody [9G11] (OACA12307)

Catalog No: OACA12307

Size:100UL

IHC image of OACA12307 diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot
Positive WB detected in: Raji whole cell lysate, K562 whole cell lysate
All lanes: INPP5D antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 134, 110 kDa
Observed band size: 145 kDa

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Recombinant Monoclonal
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay,
  Immunohistochemistry,
  Immunoprecipitation,
  Western blot
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: A synthesized peptide derived from human SHIP
• Purification: Affinity Purified

Target Info

• Gene Symbol: INPP5D
• Gene Full Name: inositol polyphosphate-5-phosphatase D
• Alias Symbols: hp51CN;Inositol polyphosphate-5-phosphatase of 145 kDa;inositol polyphosphate-5-phosphatase, 145kD;inositol polyphosphate-5-phosphatase, 145kDa;p150Ship;phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1;phosphatidylinositol 4,5-bisphosphate 5-phosphatase;SH2 domain-containing inositol 5'-phosphatase 1;SHIP;SHIP1;SHIP-1;signaling inositol polyphosphate 5 phosphatase SIP-145;signaling inositol polyphosphate phosphatase SHIP II;SIP-145.
• NCBI Gene Id: 3635
• Protein Name: Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
• Description of Target: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
• Uniprot ID: Q92835