Catalog No: OKEH02606
Size:96T
Price: $600.00
SKU
OKEH02606
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Inositol trisphosphate ELISA Kit (OKEH02606) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Inositol trisphosphate antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Inositol trisphosphate and incubated. The Inositol trisphosphate found in the sample or standards competes with the biotinylated Inositol trisphosphate for limited binding sites on the immobilized anti-Inositol trisphosphate antibody. Excess unbound biotinylated Inositol trisphosphate and sample or standard Inositol trisphosphate is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Inositol trisphosphate captured in the well and inversely proportional to the amount of Inositol trisphosphate which was contained in the sample or standard.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.3% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Inositol trisphosphate Microplate96 Wells (12 x 8 Well strips)
Inositol trisphosphate Lyophilized Standard2 x 40 ng
Sample Diluent1 x 20 mL
100X Inositol trisphosphate-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: IP3
Target Name: Inositol trisphosphate
::Pubchem: 439456
::Chemical formula: C6H15O15P3
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.265 ng/mL
SpecificityNatural and recombinant General Inositol trisphosphate
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsIP3, Inositol trisphosphate, IP3, Inositol 1, 4, 5-trisphosphate
Protein NameInositol trisphosphate
Description of TargetInositol trisphosphate or inositol 1,4,5-trisphosphate (also commonly known as triphosphoinositol; abbreviated InsP3 or IP3), together with diacylglycerol (DAG), is a secondary messenger molecule used in signal transduction and lipid signaling in biological cells. While DAG stays inside the membrane, IP3 is soluble and diffuses through the cell. It is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, by phospholipase C (PLC).
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