Catalog No: OKDD00341 (Formerly GWB-KBBCZ2)
Size:96 Wells
Price: $725.00
SKU
OKDD00341
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for INHbC ELISA Kit (Human) (OKDD00341) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||||
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Application | Enzyme-linked immunosorbent assay | ||||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||
ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology INHbC ELISA Kit (Human) (OKDD00341) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for INHbC has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for INHbC is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample INHbC captured in well. | ||||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level INHbC were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level INHbC were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10%
Inter-Assay: CV<12% | ||||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture aupernartes | ||||||||||||||||||||||||
Sensitivity | < 0.062 ng/mL | ||||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of INHbC. No significant cross-reactivity or interference between INHbC and analogues was observed. | ||||||||||||||||||||||||
Assay Info | Quantitative Sandwich ELISA |
Gene Symbol | INHBC |
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Gene Full Name | inhibin subunit beta C |
Alias Symbols | activin beta-C chain;IHBC;inhibin beta C chain;inhibin beta C subunit. |
NCBI Gene Id | 3626 |
Protein Name | Inhibin beta C chain |
Description of Target | Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. |
Uniprot ID | P55103 |
Protein Accession # | NP_005529.1 |
Nucleotide Accession # | NM_005538.3 |
Protein Size (# AA) | 352 |
Molecular Weight | 38 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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