INHBA ELISA Kit (Pig) (OKBB00669)

Catalog No: OKBB00669

Size:96 wells

INHBA ELISA Kit (Pig) (OKBB00669) Standard Curve

Product Info

• Predicted Species Reactivity: Pig
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's Pig Activin A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Activin A has been precoated onto 96-well plates. Standards(CHO, G311-S426) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Activin A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Pig Activin A amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	90	281	467	95	293	489
  Standard deviation	4.7	15.7	27.6	7.8	18.5	24.9
  CV(%)	5.2	5.6	5.9	8.2	6.3	5.1

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Pig INHBA Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Pig INHBA Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Pig INHBA Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	34	40	35	33	35	2.69	7.6%
  Sample 2	199	204	220	225	212	10.79	5%
  Sample 3	467	440	464	464	458	10.89	2.3%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.037	0.073	0.085	0.147	0.289	0.537	1.066	2.200

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Pig porcine Activin A
• Sensitivity: <12 pg/ml
• Immunogen: Expression system for standard: CHO; Immunogen sequence: G311-S426
• Description: Sandwich high sensitivity ELISA kit for Quantitative Detection of Pig porcine Activin A. 96wells/kit, with removable strips.

Target Info

• Gene Symbol: INHBA
• Gene Full Name: inhibin, beta A
• Alias Symbols: Inhibin, beta A (Activin A, activin AB alpha polypeptide) ;Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ;cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ;INHBA ;hCG_17267, tcag7.474 ;
• NCBI Gene Id: 97093
• Protein Name: Inhibin beta A chain
• Description of Target: Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
• Uniprot ID: A4D1W7
• Protein Accession #: NP_002183.1
• Nucleotide Accession #: NM_002192.3