INHBA Chemi-Luminescent ELISA Kit (Human) (OKCD03279)

Catalog No: OKCD03279

Size:96WELLS

Human INHBA ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	92-103%	87-101%	96-105%
  EDTA Plasma (n=5)	80-93%	88-95%	78-92%	99-105%
  Heparin Plasma (n=5)	89-102%	82-104%	92-99%	83-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Activin A (ACVA). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Activin A (ACVA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Activin A (ACVA) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-96	92
  EDTA Plasma (n=5)	97-105	101
  Heparin Plasma (n=5)	97-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin A (ACVA) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin A (ACVA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • INHBA Microplate: 96 Wells (12 x 8 Well strips)
  • INHBA Lyophilized Standard: 2
  • 100X INHBA Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 2.74 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: INHBA
• Gene Full Name: inhibin subunit beta A
• Alias Symbols: activin beta-A chain;EDF;erythroid differentiation factor;erythroid differentiation protein;follicle-stimulating hormone-releasing protein;FRP;FSH-releasing protein;inhibin beta A chain;inhibin beta A subunit;inhibin, beta A (activin A, activin AB alpha polypeptide);Inhibin, beta-1.
• NCBI Gene Id: 3624
• Protein Name: Inhibin beta A chain
• Description of Target: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
• Uniprot ID: P08476
• Protein Accession #: NP_002183
• Nucleotide Accession #: NM_002192