ILF2 Antibody - C-terminal region (ARP35731_T100)

Catalog#: ARP35731_T100

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: IHC, WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: ILF2
• Official Gene Full Name: Interleukin enhancer binding factor 2, 45kDa
• NCBI Gene Id: 3608
• Protein Name: Interleukin enhancer-binding factor 2
• Swissprot Id: Q12905
• Protein Accession #: NP_004506
• Nucleotide Accession #: NM_004515
• Alias Symbols: NF45, PRO3063
• Replacement Item: This antibody may replace item HPA007484
• Description of Target: Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of the interleukin 2 gene. NFAT binds to a sequence in the interleukin 2 gene enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the smaller of which is the product of the ILF2 gene. The encoded protein binds strongly to the 90 kDa protein and stimulates its ability to enhance gene expression.
• Protein Size (# AA): 390
• Molecular Weight: 43kDa
• Purification: Protein A purified
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express ILF2.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ILF2
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: HGGFRKILGQEGDASYLASEISTWDGVIVTPSEKAYEKPPEKKEGEEEEE
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: CCNDBP1; HUWE1; UBC; EEF1G; AURKA; SUMO2; SUMO3; STAU1; IVNS1ABP; RPA1; SMURF2; RPA3; RPA2; ERG; EZH2; BMI1; SUZ12; EED; rev; HNRNPU; FKBP3; DHX9; DDX1; ABCF1; C14orf166; RTCB; DIMT1; NELFB; EDC4; IGF2BP3; EIF2B2; EIF2B3; YBX3; RFC4; PTBP1; YBX1; NMT1; MR
• Blocking Peptide: For anti-ILF2 (ARP35731_T100) antibody is Catalog # AAP35731 (Previous Catalog # AAPP06977)
• Sample Type Confirmation:

  ILF2 is supported by BioGPS gene expression data to be expressed in MCF7

• Target Reference: Shin,H.J., et al., (2002) Arch. Virol. 147 (3), 471-491
• Publications:

  Jiang, D., Zhou, Y., Moxley, R. A. & Jarrett, H. W. Purification and identification of positive regulators binding to a novel element in the c-Jun promoter. Biochemistry 47, 9318-34 (2008). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 18690718

  Yamauchi, T. et al. Sepantronium bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression. Biochem. Biophys. Res. Commun. 425, 711-6 (2012). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 22842455

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: ILF2 antibody tested with Human Jurkat Cells (ARP35731_T100)

Product Protocols: ILF2 antibody tested by IHC with human kidney (ARP35731)

Product Review: ILF2 antibody - C-terminal region (ARP35731_T100) in whole cell lysates of MCF-7 WT and KO cell lines using Western Blot