IL9 ELISA Kit (Human) : 96 Wells (OKAA00013-96W)

Catalog No: OKAA00013-96W

Size:96 Wells

Standard curve of IL-9 protein using OKAA00013_96W

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Reproducibility: The coefficient of variation of both intra-assay and inter-assay were less than 10%.
• ELISA Kit Component:

  Components
  2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody
  2 vials Streptavidin-HRP solution,
  1 bottle Standard /sample Diluent
  1 bottle Biotin-Conjugate antibody Diluent
  bottle Streptavidin-HRP Diluent
  1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  1 vial Substrate Solution
  1 vial Stop Solution
  2 vials human IL-10 Standard lyophilized, 2000 pg/ml upon reconstitution
  2 vials concentrated Biotin-Conjugate anti-human IL-10 antibody

• Additional Information: Sample Type: cell culture supernates, serum, and plasma
  Reagents: REAGENTS
  1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-9 (8x12)
  2. 2 vials human IL-9 Standard lyophilized, 500 pg/ml upon reconstitution
  3. 2 vials concentrated Biotin-Conjugate anti-human IL-9 monoclonal antibody
  4. 2 vials Streptavidin-HRP solution,
  5. 1 bottle Standard /sample Diluent
  6. 1 bottle Biotin-Conjugate antibody Diluent
  7. 1 bottle Streptavidin-HRP Diluent
  8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  9. 1 vial Substrate Solution
  10. 1 vial Stop Solution
  11. 3 pieces Adhesive Films
  12. package insert
• Sample Type: cell culture, supernates, serum, and plasma
• Sensitivity: 2 pg/ml
• Predicted Homology Based on Immunogen Sequence: Human
• Specificity: This assay recognizes both natural and recombinant human IL-6. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
  Recombinant human: IL-6 sR, IL-6 sR/sgp130, IL-9, IL-10, G-CSF
  Recombinant mouse: IL-6, IL-10, IL-11, IL-12/23 p40
  Recombinant porcine: CNTF, G-CSF, IL-6, OSM
• Assay Info: Assay Method: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL9
• Gene Full Name: interleukin 9
• Alias Symbols: P40, HP40, IL-9
• NCBI Gene Id: 3578
• Protein Name: Interleukin-9
• Description of Target: The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.
• Uniprot ID: P15248
• Protein Accession #: NP_000581
• Nucleotide Accession #: NM_000590