IL9 Chemi-Luminescent ELISA Kit (Rat) (OKCD03692)

Catalog No: OKCD03692

Size:96WELLS

Rat IL9 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-99%	80-98%	82-93%	79-104%
  EDTA Plasma (n=5)	80-101%	79-104%	80-93%	85-93%
  Heparin Plasma (n=5)	78-97%	82-101%	78-95%	96-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 9 (IL9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 9 (IL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 9 (IL9) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	94-101	98
  EDTA Plasma (n=5)	78-95	86
  Heparin Plasma (n=5)	95-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 9 (IL9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 9 (IL9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Il9 Microplate: 96 Wells (12 x 8 Well strips)
  • Il9 Lyophilized Standard: 2
  • 100X Il9 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.37 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Il9
• Gene Full Name: interleukin 9
• Alias Symbols: interleukin-9.
• NCBI Gene Id: 116558
• Protein Name: Interleukin 9
• Description of Target: Interleukin 9
• Uniprot ID: D4A8I9
• Protein Accession #: NP_001099217
• Nucleotide Accession #: NM_001105747