IL8 High Sensitivity ELISA Kit (Human) (OKCD01311)

Catalog No: OKCD01311

Size:96WELLS

IL8 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	79-101%	80-95%	83-101%	80-103%
  EDTA Plasma (n=5)	92-105%	83-101%	80-89%	80-94%
  Heparin Plasma (n=5)	97-105%	78-93%	94-103%	83-99%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.9-250pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	93-101	97
  EDTA Plasma (n=5)	90-101	98
  Heparin Plasma (n=5)	89-104	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL8 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL8 Lyophilized Standard: 2
  • 100X Biotinylated IL8 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 1.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8). No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CXCL8
• Gene Full Name: C-X-C motif chemokine ligand 8
• Alias Symbols: alveolar macrophage chemotactic factor I;beta endothelial cell-derived neutrophil activating peptide;beta-thromboglobulin-like protein;chemokine (C-X-C motif) ligand 8;C-X-C motif chemokine 8;emoctakin;GCP1;GCP-1;granulocyte chemotactic protein 1;IL8;interleukin 8;interleukin-8;LECT;LUCT;lung giant cell carcinoma-derived chemotactic protein;lymphocyte derived neutrophil activating peptide;LYNAP;MDNCF;MONAP;monocyte-derived neutrophil chemotactic factor;monocyte-derived neutrophil-activating peptide;NAF;NAP1;NAP-1;neutrophil-activating peptide 1;Neutrophil-activating protein 1;Protein 3-10C;SCYB8;small inducible cytokine subfamily B, member 8;T-cell chemotactic factor;tumor necrosis factor-induced gene 1.
• NCBI Gene Id: 3576
• Protein Name: Interleukin-8
• Description of Target: IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
• Uniprot ID: p10145
• Protein Accession #: NP_000575.1
• Nucleotide Accession #: NM_000584.3