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Catalog No: OKEH04137
Size:96T
Price: $601.00
SKU
OKEH04137
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityPorcine, Sus scrofa
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL8 ELISA Kit (Pig) (OKEH04137) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL8 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 4.3% (n = 20)
Mean Inter-assay CV%: <= 9.0% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-IL8 Microplate96 Wells (12 x 8 Well Strips)
IL8 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
IL8 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity4.3 pg/mL
SpecificityNatural and recombinant Pig Interleukin-8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCXCL8
Gene Full NameC-X-C motif chemokine ligand 8
Alias Symbolsalveolar macrophage chemotactic factor I;alveolar macrophage-derived chemotactic factor-I;AMCF-I;chemokine (C-X-C motif) ligand 8;C-X-C motif chemokine 8;IL8;IL-8;interleukin-8.
NCBI Gene Id396880
Protein NameP26894
Description of TargetInterleukin-8
Uniprot IDhttps://www.uniprot.org/uniprot/P26894
Protein Accession #NP_999032.1
Nucleotide Accession #NM_213867.1
Protein Size (# AA)103
Molecular Weight12 kDa
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