IL8 Antibody (OASA07908)

Catalog No: OASA07908 (Formerly GWB-A0A76C)

Size:100UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Lyophilized. Phosphate buffered saline
• Clonality: Polyclonal
• Isotype: Polyclonal IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Functional assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
• Additional Information: Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline
  Preservative Stabilisers: None present
• Reconstitution and Storage: -20°C
• Immunogen: Recombinant human IL-8 (PHP055)
• Purification: Purified IgG prepared by affinity chromatography.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils.
  
  IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Cancer studies have demonstrated a role for IL-8 in the angiogenesis and growth of tumours. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C.
• Application Info: Suggested Dilutions:
  ELISA: 0.5-2.0ug/ml; This purified human IL-8 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated human IL-8 antibody (OASA07909) as the detection reagent and recombinant human IL-8 (OPSA10961) as the standard.
  Western Blotting: 0.1 - 0.2ug/ml; This purified human IL-8 antibody may used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-8 (OPSA10961) as the positive control.
  IHC-Paraffin:0.25-1.0 ug/ml; This product requires enzyme mediated antigen retrieval prior to staining of paraffin sections. Proteinase K is recomended for this purpose.
• Reference: 1. Mukaida, N. et al. (1989) Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8. J. Immunol. 143: 1366-1371.
  2. Srivani, R. et al. (2003) Molecular logistics of cytokines in cervical cancer. Current Science. 84: 434-438.
  

Target Info

• Gene Symbol: IL8
• Alias Symbols: IL8, NAF, GCP1, LECT, LUCT, NAP1, GCP-1, LYNAP, MDNCF, MONAP, NAP-1, SCYB8
• NCBI Gene Id: 3576
• Protein Name: Interleukin-8
• Uniprot ID: P10145
• Protein Accession #: NP_000575.1
• Protein Size (# AA): 99