IL7R Antibody : APC (OATA00070)

Catalog No: OATA00070

C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD3 (OATA00178) and 0.25 ug APC Anti-Mouse CD127 (OATA00070) (right panel) or 0.25 ug APC Rat IgG2a isotype control (left panel).

C57Bl/6 splenocytes were stained with FITC Anti-Mouse CD3 (145-2C11) and 0.25 ug APC Anti-Mouse CD127 (A7R34) manufactured by Aviva Systems Biology (left panel) or eBioscience (right panel).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: A7R34
• Isotype: Rat IgG2a, kappa
• Host: Rat
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD127 (IL-7Ra)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (Flow cytometry).

  Jin J, Goldschneider I, and Lai L. 2011. J. Immunol. 186: 1915-1922. (in vivo activation)

  Vondenhoff MF, Greuter M, Goverse G, Elewaut D, Dewint P, Ware CF, Hoorweg K, Kraal G, and Mebius RE. 2009. J. Immunol. 182(9): 5439-5445. (Immunofluorescence microscopy - frozen tissue)

  Leithauser F, Meinhardt-Krajina T, Fink K, Wotschke B, Moller P and Reimann J. 2006. Am. J. Pathol. 168(6): 1898-1909. (Immunohistochemistry - frozen tissue)

  Seddon B and Zamoyska R. 2002. J. Immunol. 169: 2997-3005. (in vivo activation)

  Sudo T, Nishikawa S, Ohno N, Akiyama N, Tamakoshi M, Yoshida H and Nishikawa S-I. 1993. Proc. Natl. Acad. Sci. 90: 9125-9129. (in vitro and in vivo blocking; Immunoprecipitation)

Target Info

• Gene Symbol: IL7R
• Gene Full Name: interleukin 7 receptor
• Alias Symbols: Il, Il17r, VDw21, Cdw217, VDw217, AW538159
• NCBI Gene Id: 16172
• Protein Name: interleukin-7 receptor subunit alpha
• Description of Target: Interleukin-7 is a glycoptorein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is a alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.
• Uniprot ID: P16872
• Protein Accession #: NP_032398.3
• Nucleotide Accession #: NM_008372.4