IL6R ELISA Kit (Pig) (OKEH08430)

Catalog No: OKEH08430

Size:96T

Pig IL6R ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology IL6R ELISA Kit (Pig) (OKEH08430) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL6R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL6R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL6R captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.6% (n = 20)
  Mean Inter-assay CV%: <=4.5% (n = 20)
• ELISA Kit Component:
  • IL6R Microplate: 96 Wells (12 x 8 Well strips)
  • IL6R Lyophilized Standard: 2
  • 100X Biotinylated IL6R Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
  Subcellular Location: Membrane Single-pass type I membrane protein
  Unigene: Ssc.16374
  SMR: O18796
  KEGG: ssc:399522
  Pfam: PF00047
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 15.7pg/mL
• Specificity: Natural and recombinant Pig Interleukin-6 receptor subunit alpha
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL6R
• Gene Full Name: interleukin 6 receptor
• Alias Symbols: IL-6 receptor alpha chain;IL-6 receptor subunit alpha;IL-6R 1;IL-6R subunit alpha;IL-6RA;IL-6Ralpha;IL-6R-alpha;interleukin-6 receptor subunit alpha.
• NCBI Gene Id: 399522
• Protein Name: O18796
• Description of Target: Interleukin-6 receptor subunit alpha
• Uniprot ID: https://www.uniprot.org/uniprot/O18796
• Protein Accession #: NP_999568.1
• Nucleotide Accession #: NM_214403.1