IL6R ELISA Kit (Human) (OKCD07685)

Catalog No: OKCD07685

Size:96WELLS

IL6R ELISA Kit (Human) (OKCD07685) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 6 Receptor (IL6R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 6 Receptor (IL6R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 6 Receptor (IL6R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 6 Receptor (IL6R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.88-120ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 Receptor (IL6R) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 Receptor (IL6R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL6R Microplate: 96 Wells (12 x 8 Well strips)
  • IL6R Lyophilized Standard: 2
  • 100X IL6R HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine, tissue homogenates and other biological fluids
• Sensitivity: < 0.67ng/mL

Target Info

• Gene Symbol: IL6R
• Gene Full Name: interleukin 6 receptor
• Alias Symbols: CD126;CD126 antigen;gp80;HIES5;IL-1Ra;IL-6 receptor subunit alpha;IL6Q;IL6QTL;IL-6R;IL-6R 1;IL-6R-1;IL6RA;IL-6RA;IL6RQ;interleukin-6 receptor subunit alpha;membrane glycoprotein 80.
• NCBI Gene Id: 3570
• Protein Name: Interleukin-6 receptor subunit alpha
• Description of Target: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
• Uniprot ID: P08887
• Protein Accession #: NP_000556.1
• Nucleotide Accession #: NM_000565.3