Size:96 Wells
$370.00
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SKU
OKAA00043-96W
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IL6 ELISA Kit (Mouse) : 96 Wells (OKAA00043-96W)

Catalog#: OKAA00043-96W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Mouse
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
Components
2 vials mouse IL-6 Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-mouse IL-6 antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminium pouches with a Microwell Plate coated with monoclonal antibody to mouse IL-6 (8x12) 2. 2 vials mouse IL-6 Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-mouse IL-6 antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
NOTE: [96 Tests]
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 7 pg/ml
Predicted Homology Based on Immunogen Sequence Mouse
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human IL-8. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IP-10, I-TAC, MIG, NAP-2, GCP-2
Recombinant mouse: KC, MIG, SDF-1a
Recombinant porcine: IL-1b
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol IL6
Official Gene Full Name interleukin 6
Alias Symbols B-cell hybridoma growth factor, Il-6, IL-6, Interleukin-6, Interleukin HP-1
NCBI Gene Id 16193
Protein Name Interleukin-6
Description of Target This gene encodes a member of the interleukin family of cytokines that have important functions in immune response, hematopoiesis, inflammation and the acute phase response. The ectopic overexpression of the encoded protein in mice results in excessive plasma cells in circulation, leading to death. Mice lacking the encoded protein exhibit abnormalities in hepatic acute phase response, some immune mechanisms, bone resorption in response to estrogen, liver regeneration and wound healing. Alternative splicing results in multiple transcript variants encoding different isoforms.
Swissprot Id P08505
Protein Accession # NP_001300983
Nucleotide Accession # NM_001314054
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IL6.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IL6.
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