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Catalog No: OKIA00192
Size:1 x 96 well plate
Price: $575.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBovine
Product Format12 of 8-well strips
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration100 Minutes
ELISA Kit PrincipleThe Aviva Systems Biology IL6 ELISA Kit (Bovine) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Bovine Interleukin-6 in Plasma or Serum. This assay employs an antibody specific for Bovine IL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and Interleukin-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-IL6 antibodies conjugated with horseradish peroxidase (HRP) are added. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. The quantity of IL6 in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
ELISA Kit Range15.63 pg/mL - 1000 pg/mL
ELISA Kit Reproducibility< 10%
ELISA Kit Component
Anti-Bovine IL6 Microplate96 Wells (12 x 8 Well Strips)
Bovine IL6 Calibrator1
100X IL6 Detection Antibody150 uL
100X Streptavidin-HRP Conjugate150 uL
5X Assay Diluent50 mL
20X Wash Solution50 mL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Additional InformationIntended Use: The IL6 test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IL6/Interleukin 6 in Bovine biological samples.
::Sample Volume: 125 uL
Reconstitution and StorageStore at 4 degrees
Sensitivity12 pg/mL
Alias SymbolsIL6;IL-6;Interleukin-6;
Protein NameInterleukin-6
Description of TargetCytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Uniprot IDP26892
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