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IL5 ELISA Kit (Human) : 48 Wells (OKAA00011-48W)

  • Catalog#: OKAA00011-48W
  • 1 week
48 Wells
$328.00
In Stock
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Predicted Species Reactivity:
Human
Application:
ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-5 (8x12)
2. 2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Gene Symbol:
IL5
Official Gene Full Name:
interleukin 5
Alias Symbols:
B-cell differentiation factor I, EDF, Eosinophil differentiation factor, IL-5, Interleukin-5, T-cell replacing factor, TRF
NCBI Gene Id:
3567
Protein Name:
Interleukin-5
Description of Target:
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Swissprot Id:
P05113
Protein Accession #:
NP_000870
Nucleotide Accession #:
NM_000879
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
2 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody
2 vials Streptavidin-HRP solution,
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL5.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL5.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human IL-4. The factors listed below were prepared at 100ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: IL-1a, IL-1ra, IL-1 sRI, IL-1 sRII, TNF-a
Recombinant mouse: IL-4
Recombinant porcine: IL-1b, TGF-b1
Assay Info:
Assay Method: Quantitative Sandwich ELISA

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