IL5 ELISA Kit (Dog) (OKCD00139)

Catalog No: OKCD00139 (Formerly GWB-KBBRE4)

Size:96WELLS

Dog IL5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Canis lupus|Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	96-104%	92-102%	99-105%	81-90%
  EDTA plasma(n=5)	94-101%	97-105%	98-105%	85-98%
  heparin plasma(n=5)	92-101%	92-101%	84-97%	81-92%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 (IL5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 5 (IL5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 (IL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 5 (IL5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	79-103	83
  EDTA plasma(n=5)	95-103	99
  heparin plasma(n=5)	94-104	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 5 (IL5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 5 (IL5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL5 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL5 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated IL5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 6.7 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 5 (IL5). No significant cross-reactivity or interference between Interleukin 5 (IL5) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL5
• Gene Full Name: interleukin 5
• Alias Symbols: eosinophil differentiation factor;IL-5;interleukin 5 (colony-stimulating factor, eosinophil);interleukin-5;T-cell replacing factor;TRF.
• NCBI Gene Id: 403790
• Protein Name: Interleukin-5
• Description of Target: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
• Uniprot ID: Q95J76
• Protein Accession #: NP_001006951
• Nucleotide Accession #: NM_001006950.1
• Protein Size (# AA): 134