IL4I1 ELISA Kit (Human) (OKCD00547)

Catalog No: OKCD00547 (Formerly GWB-KBBQA4)

Size:96WELLS

Human IL4I1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	93-102%	91-98%	85-99%	86-102%
  EDTA Plasma (n=5)	80-96%	98-105%	92-99%	96-105%
  Heparin Plasma (n=5)	89-101%	88-99%	79-101%	78-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 Induced Protein 1 (IL4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 4 Induced Protein 1 (IL4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 4 Induced Protein 1 (IL4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 4 Induced Protein 1 (IL4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-96	93
  EDTA plasma(n=5)	92-102	97
  heparin plasma(n=5)	80-98	88

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 Induced Protein 1 (IL4I1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 Induced Protein 1 (IL4I1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL4I1 Microplate: 96 Wells (12 x 8 Well Strips)
  • IL4I1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated IL4I1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.054 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 4 Induced Protein 1 (IL4I1). No significant cross-reactivity or interference between Interleukin 4 Induced Protein 1 (IL4I1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL4I1
• Gene Full Name: interleukin 4 induced 1
• Alias Symbols: FIG1;Fig-1 protein;hIL4I1;IL4-induced protein 1;interleukin four induced 1;Interleukin-4-induced protein 1;LAAO;L-amino-acid oxidase;LAO;Protein Fig-1.
• NCBI Gene Id: 259307
• Protein Name: L-amino-acid oxidase
• Description of Target: Lysosomal L-amino-acid oxidase with highest specific activity with phenylalanine. May play a role in lysosomal antigen processing and presentation (By similarity).
• Uniprot ID: Q96RQ9
• Protein Accession #: NP_001244946
• Nucleotide Accession #: NM_001258017.1
• Protein Size (# AA): 567