IL4 ELISA Kit (Monkey) (OKWB00304)

Catalog No: OKWB00304

Size:96T

Monkey IL4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Macaca fascicularis|Monkey
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-105%	85-103%	91-101%	85-98%
  EDTA Plasma (n=5)	85-99%	90-97%	82-99%	84-95%
  Heparin Plasma (n=5)	80-100%	87-100%	80-92%	84-99%

• ELISA Kit Principle: Aviva Systems Biology IL4 ELISA Kit (Monkey) (OKWB00304) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL4 captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	85-102	92
  EDTA Plasma (n=5)	85-103	93
  Heparin Plasma (n=5)	87-104	96

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • IL4 Microplate: 96 Wells (12 x 8 Well strips)
  • IL4 Lyophilized Standard: 2 x 1 ng
  • 100X IL4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL4
• Alias Symbols: B-cell stimulatory factor 1;Lymphocyte stimulatory factor 1.
• NCBI Gene Id: 103244520
• Protein Name: Interleukin-4
• Description of Target: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.
• Uniprot ID: P79339
• Protein Accession #: XP_008012578
• Nucleotide Accession #: XM_008014387