Size:96 Wells
$370.00
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SKU
OKAA00010-96W
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IL4 ELISA Kit (Human) : 96 Wells (OKAA00010-96W)

Catalog#: OKAA00010-96W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
Components
2 vials human IL-4 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-4 (8x12)
2. 2 vials human IL-4 Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 2 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human IL-3. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2, IL-4, IL-6 ,
Recombinant mouse: GM-CSF, IL-1b, IL-3, IL-5, IL-6, IL-7 ,
Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-b1, porcine TGF-b1, porcine TGF-b2 ,
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol IL4
Official Gene Full Name interleukin 4
Alias Symbols B-cell stimulatory factor 1, BCGF1, BCGF-1, Binetrakin, BSF1, BSF-1, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
NCBI Gene Id 3565
Protein Name Interleukin-4
Description of Target The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Swissprot Id P05112
Protein Accession # NP_000580
Nucleotide Accession # NM_000589
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IL4.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IL4.
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