Search Antibody, Protein, and ELISA Kit Solutions

IL4 ELISA Kit (Human) : 96 Wells (OKAA00010-96W)

  • Catalog#: OKAA00010-96W
  • 1 week
96 Wells
$370.00
In Stock
Request Bulk Order Quote

Predicted Species Reactivity:
Human
Application:
ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human IL-4 (8x12)
2. 2 vials human IL-4 Standard lyophilized, 500 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Gene Symbol:
IL4
Official Gene Full Name:
interleukin 4
Alias Symbols:
B-cell stimulatory factor 1, BCGF1, BCGF-1, Binetrakin, BSF1, BSF-1, IL-4, Interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
NCBI Gene Id:
3565
Protein Name:
Interleukin-4
Description of Target:
The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Swissprot Id:
P05112
Protein Accession #:
NP_000580
Nucleotide Accession #:
NM_000589
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
2 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human IL-4 Standard lyophilized, 1000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-4 polyclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 vials human IL-5 Standard lyophilized, 500 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human IL-5 monoclonal antibody
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL4.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human IL-3. The factors listed below were prepared at 10ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2, IL-4, IL-6 ,
Recombinant mouse: GM-CSF, IL-1b, IL-3, IL-5, IL-6, IL-7 ,
Other proteins: bovine FGF acidic, bovine FGF basic, human PDGF, porcine PDGF, human TGF-b1, porcine TGF-b1, porcine TGF-b2 ,
Assay Info:
Assay Method: Quantitative Sandwich ELISA

Product Reviews

Protocol:
Tips Information:

Tell us what you think about this item!

Write A Review
    Please, wait...