Catalog No: OKEH00129
Size:96T
Price: $601.00
SKU
OKEH00129
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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IL4 ELISA Kit (Bovine) : 96 Wells (OKEH00129)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology IL4 ELISA Kit (Bovine) (OKEH00129) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL4 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.9% (n = 20)
ELISA Kit Component
ComponentAmount
IL4 Microplate96 Wells (12 x 8 Well strips)
IL4 Lyophilized Standard2
100X Biotinylated IL4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity6.9 pg/mL
SpecificityNatural and recombinant Bovine Interleukin-4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIL4
Gene Full Nameinterleukin 4
Alias SymbolsB-cell stimulatory factor 1;BSF-1;IL-4;interleukin-4;lymphocyte stimulatory factor 1.
NCBI Gene Id280824
Protein NameP30367
Description of TargetInterleukin-4
Uniprot IDhttps://www.uniprot.org/uniprot/P30367
Protein Accession #NP_776346
Nucleotide Accession #NM_173921
Protein Size (# AA)135
Molecular Weight15 kDa
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