IL4 Chemi-Luminescent ELISA Kit (Human) (OKCD03679)

Catalog No: OKCD03679

Size:96WELLS

Human IL4 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	93-102%	82-101%	78-90%	89-97%
  EDTA Plasma (n=5)	83-95%	86-99%	93-101%	95-104%
  Heparin Plasma (n=5)	87-94%	83-101%	83-91%	84-93%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 4 (IL4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 4 (IL4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 4 (IL4) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	89-97	94
  EDTA Plasma (n=5)	78-96	87
  Heparin Plasma (n=5)	95-102	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 4 (IL4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • IL4 Microplate: 96 Wells (12 x 8 Well strips)
  • IL4 Lyophilized Standard: 2
  • 100X IL4 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 2.74 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: IL4
• Gene Full Name: interleukin 4
• Alias Symbols: B cell growth factor 1;B_cell stimulatory factor 1;B-cell stimulatory factor 1;BCGF1;BCGF-1;binetrakin;BSF1;BSF-1;IL-4;interleukin 4 variant 2;interleukin-4;lymphocyte stimulatory factor 1;pitrakinra.
• NCBI Gene Id: 3565
• Protein Name: Interleukin-4
• Description of Target: Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity).
• Uniprot ID: P05112
• Protein Accession #: NP_000580
• Nucleotide Accession #: NM_000589