IL4 Antibody : PE (OATA00379)

Catalog No: OATA00379

Human peripheral blood lymphocytes were stimulated with PMA and Ionomycin and stained with APC Anti-Human CD3 (OATA00014), followed by intracellular staining with 5 uL (0.125 ug) PE Anti-Human Il-4 (OATA00379) (right panel) or 0.125 ug PE Rat IgG1 (left pan

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: MP4-25D2
• Isotype: Rat IgG1, kappa
• Host: Rat
• Conjugation: PE: Phycoerythrin
• Application: FC
  Excitation Laser: Blue (488 nm), Green (532 nm), Yellow-Green (561 nm)
  Emmision: 578 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: IL-4
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.
• Reference:

  Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, and Silver JE. 1992. Immunol Rev. 127:5-24.

  Chretien I, Van Kimmenade A, Pearce MK, Banchereau J, and Abrams JS. 1989. J Immunol Methods. 117(1):67-71.

  Jung T, Schauer U, Rieger C, et al. 1995. Eur J Immunol. 25(8):2413-2416.

  Prussin C and Metcalfe DD. 1995. J Immunol Methods. 188(1):117-128.

Target Info

• Gene Symbol: IL4
• Gene Full Name: interleukin 4
• Alias Symbols: BSF1, IL-4, BCGF1, BSF-1, BCGF-1
• NCBI Gene Id: 3565
• Protein Name: interleukin-4
• Description of Target: The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
• Uniprot ID: P05112
• Protein Accession #: NP_000580.1
• Nucleotide Accession #: NM_000589.3