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IL4 Antibody (OASA05643)

0.5 mg
$569.00
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Gene Symbol:
IL4
NCBI Gene Id:
16189
Protein Name:
Interleukin-4
Swissprot Id:
P07750
Protein Accession #:
NP_067258.1
Alias Symbols:
IL-4, IL4
Replacement Item:
This antibody may replace item sc-1260 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI MOUSE INTERLEUKIN-4
Protein Size (# AA):
140
Host:
Rat
Clonality:
Monoclonal
Application:
IHC-AFF, ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Il4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Il4.
Immunogen:
The immunogen for anti-Il4 antibody: recombinant murine IL-4
Predicted Species Reactivity:
Mouse
Predicted Homology Based on Immunogen Sequence:
Mouse
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4C or at -20C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-Il4 antibody - OASA05643
Specificity:
Rat anti Mouse Interleukin-4 antibody, clone BVD6-24G2 recognizes mouse Interleukin-4 (IL-4). Interleukin-4 is a potent lymphoid cell growth factor, which stimulates the growth and survival of certain B and T cells.
Clone:
BVD6-24G2
Isotype:
IgG1
Application Info:
ELISA: 0.5ug/ml - 2ug/ml
Flow Cytometry: Membrane permeabilisation is required for this application. Use 10ul of the suggested working dilution to label 106cells in 100ul.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127: 5-24.
2. Bogen, S. et al. (1993) Analysis of IL-2, IL-4 and IFN gamma producing cells in situ during immune responses to protein antigens. J. Immunol. 150: 4197-4205.
3. Fujihashi, K. et al. (1993) Cytokine specific ELISPOT assay: single cell analysis of IL-2, IL-4 and IL-6 producing cells. J. Immunol. Methods 160: 181-189.
4. Ferrick, D.A. et al. (1995) Differential production of interferon-gamma and interleukin-4 in response to Th1 and Th2 stimulating pathogens by gamma/delta T cells in vivo. Nature 373: 255-257.
5. Chiu, W.C. et al. (2009) Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis.

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