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IL4 Antibody (OASA04735)

0.5 mg
$569.00
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Gene Symbol:
IL4
NCBI Gene Id:
3565
Protein Name:
Interleukin-4
Swissprot Id:
P05112
Protein Accession #:
NP_000580.1
Alias Symbols:
BSF1, IL-4, BCGF1, BCGF-1, MGC79402, IL4
Replacement Item:
This antibody may replace item sc-1260 from Santa Cruz Biotechnology.
Description of Target:
RAT ANTI HUMAN INTERLEUKIN-4
Protein Size (# AA):
153
Host:
Rat
Clonality:
Monoclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL4.
Immunogen:
The immunogen for anti-IL4 antibody: purified CHO cell expressed recombinant human IL-4.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-IL4 antibody - OASA04735
Specificity:
IL-4
Clone:
MP4-25D2
Isotype:
IgG1
Application Info:
Flow Cytometry(1):    1/10 - 1/50
Western Blotting:    1/500 - 1/1000
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(1) Special conditions apply. - See [*].
Application Data:
Application #1: Western blot analysis of human IL-4 recombinant protein probed with Rat anti Human Interleukin-4 followed by F(ab')2 Rabbit anti Rat IgG:HRP
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Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Preservative Stabilisers: 0.09% - Sodium Azide
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Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Krohn, A. et al. (1998) Staurosporine-induced apoptosis of cultured rat hippocampal neurons involves caspase-1-like proteases as upstream initiators and increased production of superoxide as a main downstream effector. J. Neurosci. 18: 8186-8197.
2. Kubota, M. et al. (1997) Oral immunization with Simian Immunodeficiency Virus p55 gag and cholera toxin elicits both mucosal IgA and systemic IgG immune responses in non human primates. J. Immunol. 158: 5321-5329.
3. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development : Immunoassay of IL-10 and IL-5 in clinical samples. Immunol. Rev. 127: 5-24.
4. Patterson, B. et al. (2001) Leukemia inhibitory factor inhibits HIV-1 replication and is upregulated in placentae from nontransmitting women. J. Clin. Invest. 107: 287-294.
5. Carvalho, E. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis. J. Immunol. 152: 5949-5956.

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