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IL4 Antibody - Biotin Conjugated (OASA00352)

0.25 mg
$549.00
In Stock
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Gene Symbol:
IL4
NCBI Gene Id:
280824
Protein Name:
Interleukin-4
Swissprot Id:
P30367
Protein Accession #:
NP_776346.1
Replacement Item:
This antibody may replace item sc-1260 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI BOVINE INTERLEUKIN-4:Biotin
Protein Size (# AA):
135
Host:
Mouse
Clonality:
Monoclonal
Application:
ELISA, FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express IL4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express IL4.
Immunogen:
The immunogen for anti-IL4 antibody: recombinant bovine IL-4
Predicted Species Reactivity:
Cow
Predicted Homology Based on Immunogen Sequence:
Cow
Product Format:
Purified IgG conjugated to Biotin - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-IL4 antibody - OASA00352
Specificity:
IL-4
Clone:
CC314
Conjugation:
Biotin
Isotype:
IgG2b
Application Info:
ELISA : This biotin conjugate may be used as detection reagent in sandwich ELISA assays for bovine IL-4 with OASA00351 as capture reagent, and recombinant bovine IL-4 (OPSA10925) as a standard.
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Additional Information:
Fusion Partners: Spleen cells form immunised BALB/c mice were fused with cells of the SP2/O myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
1% - Bovine Serum Albumin
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Hope, J.C. et al. (2005) Development of detection methods for ruminant interleukin (IL)-4. J. Immunol. Methods 301: 114-23.
2. Abbott, J.R. et al. (2005) Rapid and long-term disappearance of CD4+ T lymphocyte responses specific for

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