IL4 Antibody - C-terminal region (OAAB02658)

Catalog No: OAAB02658

Size:400ul

(LEFT)Western blot analysis of IL4 Antibody (C-term) (Cat. #OAAB02658) in WiDr cell line lysates (35ug/lane).IL4 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of IL4 Antibody (C-term) (Cat. #OAAB02658) in mouse cerebellum cell line lysates (35ug/lane).IL4(arrow) was detected using the purified Pab.

IL4 Antibody (C-term) (Cat. #OAAB02658) IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

IL4 Antibody (C-term) (Cat. #OAAB02658) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P, FC
• Additional Information: Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
  Cellular Location: Secreted.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This L4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the C-terminal region of human L4.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Monkey, Sheep
• Concentration: 0.5mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50
• Description:
  View product citations for IL4 Antibody - C-terminal region (OAAB02658) on CiteAb

Target Info

• Gene Symbol: IL4
• Alias Symbols: BSF1, IL-4, BCGF1, BSF-1, BCGF-1
• NCBI Gene Id: 3565
• Protein Name: Interleukin-4
• Description of Target: IL4 is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome.
• Uniprot ID: P05112
• Protein Accession #: NP_000580.1
• Molecular Weight: 17 kDa