IL36RN ELISA Kit (Human) (OKCD00166)

Catalog No: OKCD00166 (Formerly GWB-KBBWU6)

Size:96WELLS

Human IL36RN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	78-101%	81-95%	83-105%	85-103%
  EDTA plasma(n=5)	88-95%	90-97%	84-95%	94-102%
  heparin plasma(n=5)	94-101%	79-90%	88-96%	80-90%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Delta (FIL1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Delta (FIL1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Delta (FIL1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Interleukin 1 Delta (FIL1d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	94-105	98
  EDTA plasma(n=5)	88-101	92
  heparin plasma(n=5)	96-104	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Delta (FIL1d) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Delta (FIL1d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-IL36RN Microplate: 96 Wells (12 x 8 Well Strips)
  • IL36RN Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated IL36RN Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 1 Delta (FIL1d). No significant cross-reactivity or interference between Interleukin 1 Delta (FIL1d) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: IL36RN
• Gene Full Name: interleukin 36 receptor antagonist
• Alias Symbols: FIL1;FIL1 delta;FIL1(DELTA);FIL1D;IL-1 related protein 3;IL1F5;IL1F5 (Canonical product IL-1F5a);IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta);IL1HY1;IL1L1;IL-1ra homolog 1;IL-1-related protein 3;IL1RP3;IL-36Ra;IL36RA;interleukin 1 family, member 5 (delta);Interleukin-1 delta;Interleukin-1 family member 5;interleukin-1 HY1;interleukin-1 receptor antagonist homolog 1;interleukin-1-like protein 1;interleukin-36 receptor antagonist protein;PSORP;PSORS14.
• NCBI Gene Id: 26525
• Protein Name: Interleukin-36 receptor antagonist protein
• Description of Target: Inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor. Proposed to play a role in skin inflammation. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus. May activate an anti-inflammatory signaling pathway by recruiting SIGIRR.
• Uniprot ID: Q9UBH0
• Protein Accession #: NP_036407
• Nucleotide Accession #: NM_012275.2
• Protein Size (# AA): 155