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IL36A ELISA Kit (Mouse) (OKEH02999)

96 Wells
$469.00
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Predicted Species Reactivity:
Mouse
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology IL36A ELISA Kit (Mouse) (OKEH02999) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Il36a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Il36a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Il36a captured in well.
Kit Range:
15.6 - 1000 pg/ml
Kit Reproducibility:
Mean Intra-assay CV%: <= 6.1% (n = 20)
Mean Inter-assay CV%: <= 8.4% (n = 20)
Kit Component:
ComponentAmount
Anti-Il36a Microplate96 Wells (12 x 8 Well strips)
Il36a Lyophilized Standard2
100X Biotinylated Il36a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
IL36A
Official Gene Full Name:
interleukin 1 family, member 6
Alias Symbols:
Fil1, Fil1e, Fil1epsilon, FIL1 epsilon, Il1e, IL-1 epsilon, IL-1F6, Il1f9, Il1h1, IL-1H1, IL1RP2, Interleukin-1 epsilon, Interleukin-1 family member 6, Interleukin-1 homolog 1, MGC151479, MGC151481
NCBI Gene Id:
54448
Protein Name:
Interleukin-36 alpha
Description of Target:
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. Induces the production of proinflammatory cytokines, including IL-12, Il-1 beta, IL-6, TNF-alpha and IL-23 in bone marrow-derived dendritic cells (BMDCs). Involved in dendritic cell maturation by stimulating the surface expression of CD80, CD86 and MHC class II. Induces the production of IFN-gamma, IL-4 and IL-17 by cultured CD4+ T-cells and splenocytes. May play a role in proinflammatory effects in the lung: induces the expression of CXCL1 and CXCL2 in the lung, and the expression of TNF-alpha, IL-36c, IL-1A, IL-1B, CXCL1 and CXCL2 in isolated splenic CD11c+ alveolar macrophages. May be involved in T-cell maturation by stimulating the surface expression of CD40 and modestly CD80 and CD86 in splenic CD11c+ cells. May be involved in CD4+ T-cell proliferation. Induces NF-kappa B activation in macrophages.4 Publications
Swissprot Id:
Q9JLA2
Protein Accession #:
NP_062323.1
Nucleotide Accession #:
NM_019450.3
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
7.8 pg/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Il36a.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Il36a.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Mouse Interleukin-36 alpha
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
18 kDa
Protein Size (# AA):
160

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