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Catalog No: OKCD03670
Size:96WELLS
Price: $875.00
SKU
OKCD03670
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IL33 Chemi-Luminescent ELISA Kit (Rat) (OKCD03670)

Datasheets/ManualsPrintable datasheet for IL33 Chemi-Luminescent ELISA Kit (Rat) (OKCD03670)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)95-105%82-101%89-103%85-93%
EDTA Plasma (n=5)97-104%91-99%83-96%93-105%
Heparin Plasma (n=5)88-104%86-94%97-105%92-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 33 (IL33). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 33 (IL33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 33 (IL33) level in the sample or standard.
ELISA Kit Range1.37-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9693
EDTA Plasma (n=5)93-10197
Heparin Plasma (n=5)86-9489
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 33 (IL33) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Il33 Microplate96 Wells (12 x 8 Well strips)
Il33 Lyophilized Standard2
100X Il33 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity15.6 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolIl33
Gene Full Nameinterleukin 33
Alias SymbolsIL-33;interleukin-33;RGD1311155.
NCBI Gene Id361749
Protein NameInterleukin-33
Description of TargetCytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (By similarity).
Uniprot IDQ66H70
Protein Accession #NP_001014188
Nucleotide Accession #NM_001014166
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